Characterization of Keratinocytes, Fibroblasts and Melanocytes Isolated from Human Skin using Gene Markers

Cells isolated from skin have wide applications in studies of the pathogenesis skin-related diseases and construction 3D equivalents. This study aimed to isolate keratinocytes, fibroblasts, melanocytes human foreskin characterize purity cell types. Keratinocytes, were by differential trypsinization media selection. The types was characterized based on expression gene markers. assessment marker involved RNA extraction, primer design, quantitative polymerase chain reaction (qPCR) immunocytochemical staining. Our results showed that cocultures keratinocytes fibroblasts dermis, could be separated quick culture Dulbecco’s modified Eagle’s medium. remaining are cultured Epilife Melanocytes epidermis selected changing medium M254 Gene suggested cytokeratin 14 (CK14) is a suitable for elastin (ELN) tyrosinase (TYR) tyrosinase-related proteins 1 2 (TYRP1 TYRP2) markers melanocytes. In conclusion, can same sample selection techniques finding will aid isolation pure various regenerative medicine toxicity studies.